FIG. 5.

IEM of negatively stained TN106 showing the binding of antiserum to NucA. Binding was detected with goat anti-mouse antiserum conjugated with 12-nm gold spheres, and the cells were stained with vanadium. Mouse polyclonal anti-NucA serum was preabsorbed with a whole-cell nucA knockout mutant to reduce background. Bar, 100 nm.