TABLE 1.
Primers used in this study
| Primer | Positiona | Sequenceb |
|---|---|---|
| 63K P1 | 76–99 | AAAGAAGC(AT)CC(AT)CAAGC(AT)CA(TC)AAA |
| 63K P2 | 115–138 | AT(TC)TTACA(TC)AT(TC)AATGATCA(TC)CAT |
| 4101 Ext | 183–202 | GCAAACCAAAGTGGATATTG |
| 4102 Ext | 607–589 | CATCATAGAACTTCACATC |
| 4313 Ext | 1534–1551 | GTGAGTGTTGAAGTCTTG |
| 63K BamRv | 1852–1836 | [GCTACGGGATCC]GCGCGTTAAATACGCGC |
| NcoF63K | 1–27 | [ACCATGGGT]ATGCTTTTATCCAAAAAATCAGCTACC |
| Blue T3 | ATTAACCCTCACTAAAGGGA | |
| Blue T7 | CGACTCACTATAGGGAGACC |
a
Locations of primer positions are based on the nucA DNA sequence shown in Fig. 3.
b
Bases in bold are changes from the genomic sequence. Bases in parentheses are wobbled for the bases shown. Bases in angle brackets are tailed bases added for cloning. Underlined bases denote a restriction sequence.