TABLE 7.
Substrate specificity of 5′-nucleotidase activitya
Substrate | Relative activityb of:
|
|
---|---|---|
NucA | rNucA | |
AMP | 100 | 100 |
ADP | 89 | 40 |
ATP | 81 | 97 |
CMP | 91 | 106 |
CDP | 92 | 95 |
CTP | 59 | 97 |
GMP | 94 | 96 |
GDP | 73 | 39 |
GTP | 75 | 37 |
TMP | 124 | 133 |
TDP | 72 | 10 |
TTP | 73 | 26 |
UMP | 81 | 62 |
UDP | 84 | 72 |
UTP | 53 | 103 |
5′-Nucleotidase was assayed as described in Materials and Methods. Substrates were added to a final concentration of 1.5 mM.
Values are expressed as percent relative activity compared to 5′AMP, which is given a value of 100.