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. 2022 Nov 29;13:1056190. doi: 10.3389/fpls.2022.1056190

Figure 2.

Figure 2

T-DNA delivery into relevant tissues of maize SEEs by centrifugation during inoculation. (A) Agrobacterium inoculation by centrifugation at 1 x g with visual light control for GFP; (B) Agrobacterium inoculation by centrifugation at 291 x g; (C) GFP expression after Agrobacterium inoculation by centrifugation at 1 x g; (D) GFP expression after Agrobacterium inoculation by centrifugation at 291 x g; (E) GUS expression after Agrobacterium inoculation by centrifugation at 1 x g; (F) GUS expression after Agrobacterium inoculation by centrifugation 291 x g; (G) Detailed view of GFP expression of a SEE inoculated by centrifugation in the presence of Agrobacterium, under a confocal microscope, LP-leaf primordia, SAM-shoot apical meristem. pMON42073 ( Supplemental Figure S1 ) was used for these experiments.