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. 2022 Nov 29;13:1056190. doi: 10.3389/fpls.2022.1056190

Figure 4.

Figure 4

Regeneration of fertile events from multiple buds. (A) SEEs after co-culture for 3-6 days; (B) Bud formation from SEEs on bud induction medium 1484 containing 4.0 mg/L BAP and 2.2 mg/L picloram for two weeks; (C) A GFP positive bud formation from SEEs cultured on1484 medium containing 4.0 mg/L BAP and 2.2 mg/L picloram; (D) Elongating buds expressing GFP; (E) Buds induced on induction medium were transferred to bud proliferation medium 2913 containing 1.0 mg/L 2,4-D, 2.2mg/L picloram, and 25 µM glyphosate for 4 weeks; (F) Regeneration of transgenic plants 2-4 weeks after transfer of resistant buds to regeneration medium 1083 containing 25 µM glyphosate; (G) Leaf from regenerated plants expressing GUS; (H) Transgenic plants in soil pots in greenhouse; (I) Normal silk formation; (J) Normal tassel formation; (K) GUS assay of pollen to confirm germline transformation; (L) Normal seed set on transgenic event.