Figure 8.

Reduction of chimeric event through the introduction of the extended bud proliferation step with selection and optimized hormone combination. (A, B) proliferation of glyphosate-resistant shoot buds on bud induction medium 4141 containing 3 mg/L thidiazuron, 2 mg/L picloram, and 30 mM glyphosate for two weeks; (C) Regeneration of glyphosate-resistant shoots on regeneration medium containing 20 mM glyphosate; (D) Glyphosate-resistant shoots developed a strong root system on rooting medium; (E) Transgenic plants with normal green leaf phenotype in plug; (F) gusA expression in non-chimeric glyphosate-resistant shoots; (G) Bud proliferation induction medium significantly (p<0.01) reduced the regeneration of chimeric shoots; (H) bud proliferation medium significantly (p<0.01) improved the overall production of non-chimeric events. ** indicates significance at <0.01 level.