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ITC experiments to assess LPS binding by PmxB4/ent-PmxB4 and PMBN/ent-PMBN. (A) Representative thermograms resulting from titration of the polymyxin peptide indicated (1 mM) into the LPS solution (20 μM). (B) Observed enthalpy change upon titration of peptide into LPS. All experiments were run in triplicate; full data provided in Figures S8–S14 and Table S5.
