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Oligosaccharide fractionation of (A) hydrolyzed IbSF (0.05 mol/dm3 H2SO4 at 60 °C for 9 h), and (B) hydrolyzed HfSF (0.01 mol/dm3 HCl at 60 °C for 5 h) in a Bio-Gel P-10 column. Fractions (1 cm3 each) were eluted by aqueous 10% ethanol solution containing 1.0 mol/dm3 NaCl and detected by metachromasy using 1,9-dimethylmethylene blue with absorbance at 525 nm. Fractions (Fr 1- Fr 5 for IbSF, and Fr 1-Fr 6 for HfSF) were pooled as indicated in the panels.
