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. 2022 Dec 6;10:e14525. doi: 10.7717/peerj.14525

Table 3. Summary statistics of each of the eight assemblies analyzed, with distinct values of key parameters including the minimum depth to keep a base call in the consensus sequence (–d) and the minimum coverage of a sequence to be included in the final alignment.

Key parameters Loci (m, r) Terminals Aligned (bp) Variable sites PIS Missing data (%) Bootstrap (mean)
–d 2 –C 0.7 120 [1, 313] 13 540,764 118,386 16,592 63.3 66.9
–d 2 –C 0.5 268 [8, 638] 15 1,064,863 261,671 48,638 56.2 84
–d 2 –C 0.3 423 [18, 682] 16 1,442,185 411,563 93,974 46.1 97.3
–d 2 –C 0.1 618 [286, 683] 16 1,494,809 449,182 110,495 41.1 94.4
–d 3 –C 0.1 490 [39, 683] 16 1,257,452 332,727 69,297 50.6 99.1
–d 4 –C 0.1 382 [8, 672] 16 932,497 217,549 37,358 58 95.5
–d 5 –C 0.1 287 [1, 586] 16 537,866 115,958 17,078 62.3 87.3

Note:

Loci (m, r), mean and range number of recovered loci (at least partially) across all terminals; Terminals, Number of terminals in the concatenated alignment; Bootstrap (mean), mean boostrap in the concatenated phylogeny (including the same 13 terminals).