Table 3. Summary statistics of each of the eight assemblies analyzed, with distinct values of key parameters including the minimum depth to keep a base call in the consensus sequence (–d) and the minimum coverage of a sequence to be included in the final alignment.
Key parameters | Loci (m, r) | Terminals | Aligned (bp) | Variable sites | PIS | Missing data (%) | Bootstrap (mean) |
---|---|---|---|---|---|---|---|
–d 2 –C 0.7 | 120 [1, 313] | 13 | 540,764 | 118,386 | 16,592 | 63.3 | 66.9 |
–d 2 –C 0.5 | 268 [8, 638] | 15 | 1,064,863 | 261,671 | 48,638 | 56.2 | 84 |
–d 2 –C 0.3 | 423 [18, 682] | 16 | 1,442,185 | 411,563 | 93,974 | 46.1 | 97.3 |
–d 2 –C 0.1 | 618 [286, 683] | 16 | 1,494,809 | 449,182 | 110,495 | 41.1 | 94.4 |
–d 3 –C 0.1 | 490 [39, 683] | 16 | 1,257,452 | 332,727 | 69,297 | 50.6 | 99.1 |
–d 4 –C 0.1 | 382 [8, 672] | 16 | 932,497 | 217,549 | 37,358 | 58 | 95.5 |
–d 5 –C 0.1 | 287 [1, 586] | 16 | 537,866 | 115,958 | 17,078 | 62.3 | 87.3 |
Note:
Loci (m, r), mean and range number of recovered loci (at least partially) across all terminals; Terminals, Number of terminals in the concatenated alignment; Bootstrap (mean), mean boostrap in the concatenated phylogeny (including the same 13 terminals).