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Immunoblot of recombinant OmpA. Recombinant OmpA (lane 1 of each panel) and lysates of E. coli O18:K1:H7 bacteria (lane 2 of each panel) were electrophoresed on an SDS–16% polyacrylamide gel and transferred to nitrocellulose. Primary antibodies included polyclonal mouse anti-J5 IgG (left) and the monoclonal antibody directed against the 35-kDa OMP (2D3; right).
