Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 12;113(12):4120–4134. doi: 10.1111/cas.15571

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

PMC Copyright notice

Interleukin‐8 (IL‐8) promotes metastasis of liver cancer and predicts patient outcomes. (A) Comparison of serum interleukin‐8 (IL‐8) expression levels between hepatocellular carcinoma (HCC) metastatic and nonmetastatic groups (p = 0.05). (B) HCC patients with low IL‐8 expression had better OS. (C) Expression of Snail, Twist1, N‐cadherin, fibronectin, and vimentin increased, whereas E‐cadherin and ZO‐1 decreased in liver cancer cells treated with rh‐IL‐8. (D) A spike‐like filopodia morphology change was observed in IL‐8‐stimulated cells. Cells were stained with F‐actin (green) and counterstained with Hoechst (blue). Magnification, ×400. (E) Wound healing and Transwell assays in Huh‐7 and HepG2 cells. IL‐8 stimulation enhanced liver cancer cell migration and invasion abilities. (F) Mice treated with IL‐8 showed more liver metastatic nodules and high expression of Snail, Twist1, and vimentin, whereas E‐cadherin was downregulated. Huh‐7 cells were used in the HCC metastasis model with or without IL‐8 intraperitoneal injection (100 ng per mouse, once a week), n = 6. Values are expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.