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. 2022 Oct 12;113(12):4120–4134. doi: 10.1111/cas.15571

FIGURE 3.

FIGURE 3

MicroRNA (miR)‐370‐3p inhibits the migration and invasion of liver cancer cells in vitro and in vivo. (A) Real‐time PCR quantification of Snail and Twist1 expression in Huh‐7 and HepG2 cells transfected with miR‐370‐3p mimic or inhibitor for 48 h. mRNA expression was normalized to β‐actin. (B) Transfection of miR‐370‐3p mimic inhibited Snail and Twist1 at the protein level in Huh‐7 and HepG2 cells. At the same time, E‐cadherin and ZO‐1 increased, whereas the expression of N‐cadherin, fibronectin, and vimentin decreased. However, miR‐370‐3p inhibitor exerted opposite effects. β‐Tubulin was used as a loading control. (C) miR‐370‐3p inhibited Huh‐7 and HepG2 cell migration and invasion. (D) Mice bearing Huh‐7miR‐370‐3pmimic showed much less liver metastasis than control with two‐tailed Student's t‐test, n = 6. Experiments were performed in triplicate, and data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.