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. 2022 Oct 12;113(12):4120–4134. doi: 10.1111/cas.15571

FIGURE 5.

FIGURE 5

Interleukin‐8 (IL‐8) suppressed microRNA (miR)‐370‐3p expression by signal transducer and activator of transcription 3 (STAT3) occupying the miR‐370‐3p promoter. (A) Expression of miR‐370‐3p was dramatically decreased after IL‐8 treatment. (B) Spearman's correlation analysis indicated that the STAT3 level was positively correlated with IL‐8 level in The Cancer Genome Atlas database. (D) miR‐370‐3p levels were rescued by Stattic after IL‐8 treatment. (D) Stattic attenuated the promoting effects exerted by IL‐8 on Huh‐7 and HepG2 cell migration and invasion. (E) Schematic illustration of miR‐370‐3p promoter with the indicated STAT3‐binding capability. (F) HEK293T cells were cotransfected with miR‐370‐3p‐luc reporter and STAT3 for 48 h and then subjected to luciferase activity assay. STAT3 suppressed miR‐370‐3p promoter activity. (G) Direct interaction between STAT3 and miR‐370‐3p promoter was confirmed by ChIP assays. *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001.