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. 2022 Oct 12;113(12):4120–4134. doi: 10.1111/cas.15571

FIGURE 6.

FIGURE 6

Signal transducer and activator of transcription 3 (STAT3) recruits histone deacetylase 1 (HDAC1) and inhibits microRNA (miR)‐370‐3p promoter activity (A) HEK293T cells were cotransfected with Myc‐STAT3 and Flag‐HDACs for 48 h, then lysed and analyzed by immunoprecipitation (IP). (B) After transfection of siHDACs, the expression of miR‐370‐3p were examined by quantitative RT‐PCR. (C) HEK293T cells were cotransfected with miR‐370‐3p‐Luc reporter and siHDACs for 48 h, and the luciferase activity assay showed that siHDAC1 significantly increased miR‐370‐3p promoter luciferase activity. (D) HDAC inhibitors (SAHA and Chidamide) increased miR‐370‐3p expression. (E) SAHA inhibited Huh‐7 and HepG2 cell migration and invasion. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. ns, not significant.