FIGURE 2.
Flow cytometric analysis of cellular uptake. Population of Alexa647‐labeled siRNA‐encapsulated PGlu(DET‐CAR)30 LNP in SKOV3‐luc cells with (A) a 2 h incubation and (B) a 6 h incubation. Population of Alexa647‐labeled siRNA‐encapsulated PGlu(DET‐CAR)30 LNP in CT 26 cells with (C) a 2 h incubation and (D) a 6 h incubation. Quantification analysis of cellular uptake in (E) SKOV3‐luc cells and (F) CT26 cells. In total, 3000 events were acquired for each sample. The results are shown as the mean ± SD (n = 3), **** p < 0.0001 (Student's t‐test)