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. 2022 Sep 15;113(12):4104–4119. doi: 10.1111/cas.15551

FIGURE 5.

FIGURE 5

Knocking down SAR1A induces reactive oxygen species (ROS) generation and endoplasmic reticulum (ER) stress. (A, B) SAR1A knockdown resulted in reductions in the expression of GRP78, SOD, and CAT together with increases in the levels of ER stress markers (p‐eIF2α, ATF4, and CHOP). (C, D) ROS fluorescence probe was used to detect ROS content in osteosarcoma cells after SAR1A knockdown or N‐acetylcysteine (NAC) pretreatment (10 mmol/L for 12 h). Analyses were repeated three times. shNC, normal control. * p <0.05 (mean ± SD).