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. Author manuscript; available in PMC: 2022 Dec 13.
Published in final edited form as: J Mol Biol. 2018 Jan 31;430(5):641–654. doi: 10.1016/j.jmb.2018.01.003

Fig. 2.

Fig. 2.

Transcription activity of rUAF. (a). Increasing amounts of rUAF (5, 10, and 20 nM) were added to transcription competent wild-type (WT) yeast extracts. Pol I primer extension products were analyzed by a Urea containing SDS-PAGE gel. (b). rUAF (20 nM) was added to Δrrn5 transcription competent yeast extracts. Activity assays were performed in duplicate and representative assay results are shown. Primer extension products are denoted with an asterisk to the right of the gel image. Band intensities of primer extension products are depicted below the gel images as a bar graph. Relative activities (Rel. Act.) were normalized to the activity in absence of rUAF, which was set at 1.0. Error bars denote standard deviation. Transcription activity assays were performed in duplicate and representative assay results are shown. Error bars denote standard deviation.