Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 13;23:256. doi: 10.1186/s13059-022-02824-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Timeline, type, and specifications of spatial omics technologies. A Timeline of all super resolution methodologies from 2010. Color intensity corresponds to the number of technologies published each year, and blue colored techniques are sequencing-based while red colored techniques are multiplexed IHC/IF methodologies. Top and bottom of the red boxes represent non-FISH and FISH based, respectively. B Proportions of target types and analysis approaches, where Y quantifies methods detecting given analyte (DNA, RNA, or protein). C Comparison of imaging-based technologies. Highest resolution that can be done, some of the super resolution microscopy-based methods were estimated at 50 nm and maximum number of the markers or genes that can be detected in an experiment. Note that depending on the technology, some of them were not optimized yet and can be expanded in future years. Excluded seqFISH + and MERFISH which claims up to 10,000 markers for detection (probe-based method)