Table 4.
A short-list of putative candidate genes related to Pro and H2O2 accumulation under drought stress and theSTI. The genes overlap with the significant markers or haplotype block regions
| Trait | Marker/Haplotype | Chr | Position (bp) | Candidate gene | Protein | Gene OntolGene Ontology |
|---|---|---|---|---|---|---|
| Pro_dro | AX-158569423 | 1A | 8,248,738 | TraesCS1A01G015300 | Ras-like protein | GO:0005525 MF: GTP binding |
| BS00009970_51 | 4A | 45,338,226 | TraesCS4A01G053700 | CaM_binding domain-containing protein | ||
| Pro_1A_Hap1 | 1A | 7,835,834 to 8,239,960 | TraesCS1A01G014400 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | |
| TraesCS1A01G014500 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | ||||
| TraesCS1A01G014900 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | ||||
| TraesCS1A01G015000 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | ||||
| Pro_1A_Hap2 | 1A | 33,365,205 to 33,375,641 | TraesCS1A01G051900 | transmembrane protein | NA | |
| Pro_1A_Hap3 | 1A | 586,717,462 to 586,915,440 | TraesCS1A01G051000 | F-box protein | NA | |
| TraesCS1A01G051100 | F-box/LRR-repeat protein | NA | ||||
| TraesCS1A01G051600 | F-box family protein | GO:0005515 MF: protein binding | ||||
| Pro_3B_Hap1 | 3B | 18,820,910 to18822191 | TraesCS3B01G039100 | MYB transcription factor | GO:0003677 MF: DNA binding | |
| TraesCS3B01G039200 | NBS-LRR disease resistance protein | GO:0043531 MF: ADP binding | ||||
| Pro_7B_Hap1 | 7B | 144,074,930 to 144,705,984 | TraesCS7B01G122600 | Aquaporin | GO:0005215 MF: transporter activity | |
| TraesCS7B01G122800 | Receptor-like protein kinase | GO:0004672 MF: protein kinase activity | ||||
| TraesCS7B01G122900 | Zinc finger family protein | GO:0003676 MF: nucleic acid binding | ||||
| Pro_6D_Hap1 | 6D | 470,391,193 to 470,683,534 | TraesCS6D01G401100 | Pentatricopeptide repeat-containing protein | GO:0005515 MF: protein binding | |
| Pro_4A_Hap1 | 4A | 40,442,320 to 43,031,174 | TraesCS4A01G049900 | Protein kinase | GO:0004672 MF: protein kinase activity | |
| TraesCS4A01G050000 | Phosphatase 2C family protein | GO:0003824 MF: catalytic activity | ||||
| TraesCS4A01G050100 | Tetratricopeptide repeat protein 1 | GO:0005515 MF: protein binding | ||||
| TraesCS4A01G050400 | Pentatricopeptide repeat-containing protein, putative | GO:0005515 MF: protein binding | ||||
| TraesCS4A01G050500 | Metal transporter | GO:0005215 MF: transporter activity | ||||
| Pro_STI | AX-110412102 | 2A | 775,936,337 | TraesCS2A01G584400 | WAT1-related protein | GO:0016020 CC: membrane |
| Ku_c1575_338 | 3B | 726,481,592 | TraesCS3B01G475800 | Auxin response factor | GO:0003677 MF: DNA binding | |
| AX-158550762 | 5A | 451,456,903 | TraesCS5A01G235300 | Hexosyltransferase | GO:0016758 | |
| AX-158525047 | 5B | 490,619,499 | TraesCS5B01G307200 | DNA polymerase delta subunit 4 | GO:0006260 BP: DNA replication | |
| GENE_3437_148 | 5B | 489,280,672 | TraesCS5B01G305100 | PF02181: Formin Homology 2 Domain | G | |
| Jagger_c3991_101 | 5B | 488,820,722 | TraesCS5B01G304800 | cytochrome P450, family 702, subfamily A, polypeptide 6 | NA | |
| Excalibur_c9846_458 | 5B | 505,482,207 to 506,269,971 | TraesCS5B01G320600 | Pentatricopeptide repeat-containing protein | GO:0005515 MF: protein binding | |
| Pro_3B_Hap4 | 3B | TraesCS3B01G475800 | Auxin response factor | GO:0003677 MF: DNA binding | ||
| TraesCS3B01G476500 | F-box protein | NA | ||||
| TraesCS3B01G476700 | Auxin response factor | NA | ||||
| TraesCS3B01G476800 | Prefoldin subunit 5 | NA | ||||
| TraesCS3B01G476900 | F-box protein family | GO:0005515 MF: protein binding | ||||
| TraesCS3B01G477000 | E3 ubiquitin protein ligase drip2 | NA | ||||
| Pro_4B_Hap1 | 4B | TraesCS4B01G292700 | Transmembrane protein, putative | NA | ||
| TraesCS4B01G292900 | AP2-like ethylene-responsive transcription factor | GO:0003677 MF: DNA binding | ||||
| Pro_5A_Hap1 | 5A | TraesCS5A01G516800 | Basic-leucine zipper (bZIP) transcription factor family protein | NA | ||
| Pro_5B_Hap1 | 5B | TraesCS5B01G320600 | Pentatricopeptide repeat-containing protein | GO:0005515 MF: protein binding | ||
| Pro_5B_Hap2 | 5B | TraesCS5B01G321800 | Uncharacterized protein | GO:0005524 MF: ATP binding | ||
| Pro_5B_Hap3 | 5B | TraesCS5B01G387400 | F-box family protein | GO:0005515 MF: protein binding | ||
| H2O2_STI | HP_1B_Hap1 | 1B | TraesCS1B01G007800 | TAF domain-containing protein | GO:0046982 MF: protein heterodimerization | |
| AX-86183817 | 1B | 614,452,475 | TraesCS1B01G382000 | Lys-63-specific deubiquitinase BRCC36 | GO:0005515 MF: protein binding | |
| AX-158596005 | 2A | 751,004,758 | TraesCS2A01G537100 | Superoxide dismutase | GO:0004784 MF: superoxide dismutase activity | |
| Excalibur_c9752_289 | 2B | 760,950,856 | TraesCS2B01G570500 | phosphatidylinositol 4-kinase gamma-like protein | GO:0016301 MF: kinase activity | |
| HP _2B_Hap1 | 2B | 51,928,949 to 73,198,699 | TraesCS2B01G090200 | F-box family protein | GO:0005515 MF: protein binding | |
| TraesCS2B01G091500 | Cytochrome P450 | GO:0005506 MF: iron ion binding | ||||
| TraesCS2B01G566900 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | ||||
| HP _2B_Hap2 | 2B | 758,593,004 to 760,933,257 | TraesCS2B01G567000 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | |
| TraesCS2B01G567100 | Serine/threonine-protein kinase | GO:0004674 MF: protein serine/threonine kinase activity | ||||
| TraesCS2B01G567500 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | ||||
| TraesCS2B01G567600 | Superoxide dismutase | GO:0004784 MF: superoxide dismutase activity | ||||
| TraesCS2B01G567700 | Serine/threonine-protein kinase | GO:0004674 MF: protein serine/threonine kinase activity | ||||
| TraesCS2B01G567800 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | ||||
| TraesCS2B01G568000 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | ||||
| TraesCS2B01G568500 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | ||||
| TraesCS2B01G568600 | Serine/threonine-protein kinase | GO:0004672 MF: protein kinase activity | ||||
| HP _6D_Hap1 | 6D | 3,119,406 to 3,141,405 | TraesCS6D01G007800 | receptor kinase 1 | GO:0004672 MF: protein kinase activity | |
| TraesCS6B01G138800 | F-box plant-like protein, putative | NA | ||||
| TraesCS6B01G138900 | F-box plant-like protein, putative | NA | ||||
| H2O2_dro | AX-158557366 | 2A | 749,105,336 | TraesCS2A01G533200 | Flavin-containing monooxygenase | GO:0016491 MF: oxidoreductase activity |
Abbreviations: MF, molecular function; Pro_dro, Proline content under drought conditions; H2O2_dro, H2O2 content under drought conditions