TABLE 3.
The genes of bal BGC and their annotated functionsa
| Gene | AA | Closest homolog | UniProt ID | % Identity/% similarity | Predicted function | Homologs in sim and other angucycline BGCsb |
|---|---|---|---|---|---|---|
| balA7 | 485 | Hydroxylase/dehydrase | A0A1M5TK19 | 90/93 | Flavin monooxygenase | simA7, lanE, sqnF, sprB, may8, bexE |
| balA4 | 109 | Polyketide cyclase | A0A1M5TKA0 | 91/96 | Polyketide cyclase | simA4, lanF, jadI, sprC, may17, bexF |
| balA1 | 420 | PKS ketosynthase | A0A1M5TK21 | 97/98 | Polyketide ketosynthase | simA1, lanA, sprD, sqnH, jadA, bexA |
| balA2 | 404 | Beta-ketoacyl synthase | A0A101ULX5 | 92/95 | Polyketide ketosynthase | simA2, lanB, sim3, sprE, bexB |
| balA3 | 89 | ACP | A0A1M5TK95 | 81/86 | ACP | simA3, lanC, sqnJ, sprF, bexC |
| balA6 | 261 | Ketoacyl reductase | A0A117RWC5 | 97/98 | Ketoreductase | simA6, lanD, sprG, sqnK, bexD |
| balA5 | 314 | Polyketide cyclase | A0A117RWC7 | 92/95 | Cyclase | simA5, lanL, sim6, spr H, sqnL, bexL |
| balA8 | 513 | Oxygenase | A0A1M5TKE0 | 84/88 | Flavin monooxygenase | simA8, lanM, sim7, sqnM, sprB, bexE |
| balA9 | 253 | Short-chain dehydrogenase/reductase | A0A1M5TKH0 | 90/96 | Oxygenase/reductase | simA9, lanV, sim8, sprI, sqnM, bexM |
| balEx | 486 | MFS domain protein | A0A117RWH8 | 75/80 | Transporter | sqnN, sprJ, saqJ, BexJ |
| balA10 | 191 | Flavin reductase | A0A1M5TL23 | 87/92 | Flavin reductase | simA10, lanZ4, sim9, saqO, sqnC, urdO |
| balA11 | 164 | Acyl-CoA carboxylase | A0A101UMQ2 | 66/70 | Unknown | |
| balB7 | 381 | C-glycosyltransferase | A0A117RWK8 | 88/92 | C-glycosyltransferase | simB7, lanGT2, sqnG3, saqGT5, sprGT3 |
| balB1 | 355 | Thymidylyltransferase | A0A1M5TM58 | 88/93 | Thymidylyltransferase | lanG, sqnS2, saqG, sprL |
| balB4 | 359 | Dehydrogenase | A0A1M5TM89 | 75/82 | Oxidoreductase | simB4, lanT, saqT, sqnS8, sprS |
| balB2 | 327 | dTDP-glucose 4,6-dehydratase | A0A101ULU7 | 88/93 | NDP-hexose 4,6-dehydratase | lanH14, sqnS3, saqH, sprM |
| balB5 | 321 | Epimerase | A0A1M5TLG1 | 79/84 | NDP-hexose 4-ketoreductase | simB5, lanR, saqR, sqnS6, sprP |
| balB3 | 462 | NDP-hexose 2,3-dehydratase | A0A101ULW8 | 84/88 | NDP-hexose 2,3-dehydratase | simB3 lanS, sim20, sqnS7, sprR |
| balC3 | 338 | 3-oxo-ACP synthase | A0A1M5TLT0 | 85/92 | 3-oxo-ACP synthase | simX5 |
| balC8 | 270 | Stilbenecarboxylate synthase | A0A101ULZ3 | 78/84 | Type III PKS synthase | |
| balA12 | 544 | Carboxyltransferase | A0A101UM08 | 91/95 | Decarboxylase | simA12, lanP, sqnP, sprU, BexH |
| balC7 | 88 | ACP | A0A1M5TLZ2 | 87/94 | ACP | simorf1 |
| balC2 | 418 | Ketosynthase | A0A1M5TLW4 | 92/95 | 3-oxo-ACP synthase II | simorf3, lanA, sqnH, saqA, sprD |
| balC1 | 360 | Ketosynthease | A0A1M5TLP8 | 85/92 | 3-oxo-ACP synthase II | simorf2 |
| balC6 | 248 | 3-oxoacyl-ACP reductase | A0A1M5TLW7 | 86/93 | 3-oxo-ACP-reductase | simD4, simJ2, sqnM |
| balE | 319 | Histidine kinase | A0A101ULU1 | 75/82 | Histidine kinase | |
| balReg1 | 220 | Response regulator | A0A1M5TLZ1 | 92/93 | Transcriptional regulator | simReg1, sim1, sqnR, sprR3 |
| balF | 163 | TIR domain-containing protein | A0A0M9YDF9 | 58/91 | Hypothetical protein |
a
Genes responsible for the biosynthesis of the angucycline aglycone, deoxysugar and unsaturated fatty acid moieties are shaded in red, brown and green, respectively.
b
Sim, simocyclinone BGC; Urd, urdamycin BGC; Lan, landomycin BGC; Sqn, saquayamycin BGC; Spr, saprolmycin BGC; May, mayamycin BGC; Bex, BE-7585A BGC; Jad, jadomycin BGC.