TABLE 3.
Properties of the C. jejuni 81116 galE mutant virulence indicators compared to those of the parent wild-type strain
| Strain | % Adherencea | % Invasiona | % Survivalb | Hemolysis (% of lysed sheep erythrocytes)
|
% DNA uptakec | ||
|---|---|---|---|---|---|---|---|
| 4 h | 17 h | 41 h | |||||
| Wild type | 100 | 100 | 25.6 ± 12.3 | 7.7 ± 2.0 | 34.7 ± 22.2 | 61 ± 8.9 | 100 |
| galE mutant | 4.6 ± 5.2 | 0.9 ± 1.2 | 24.1 ± 14.3 | 8.5 ± 1.3 | 46.3 ± 7.2 | 86.9 ± 26.4 | 4.5 ± 5.1 |
a
Adherence or invasion of INT407 cells by C. jejuni 81116 galE mutant compared to the parent strain C. jejuni 81116. The number of 81116 cells that adhered or invaded was taken as 100%.
b
Percentage of surviving cells after incubation for 30 min with NHS.
c
Transformation efficiency (calculated per microgram of DNA per 108 CFU) compared to the transformation efficiency of 81116.