Table 1.

Experimental variables and responses used in the mixture design

	Variables			TPC (mg GAE/mL)		TFC (mg QE/mL)		MIC S. aureus (mg/mL)		MIC S. enterica (mg/mL)		MIC E. coli (mg/mL)		DPPH (IC 50) (mg/mL)
Runs	Water X1	Ethanol X2	Acetonitrile X3	Exp	Pred	Exp	Pred	Exp	Pred	Exp	Pred	Exp	Pred	Exp	Pred
1	1.000	0.000	0.000	$44.84\pm 2.24b$	44.915	$0.222\pm 0.011$ b	0.244	$2.500\pm 0.125$ c	2.352	$1.250\pm 0.063$ c	1.150	$0.625\pm 0.031$ c	0.586	$1.225\pm 0.061$ d	1.134
2	0.000	1.000	0.000	$42.32\pm 2.12$ a	41.242	$0.289\pm 0.014$ d	0.275	$0.625\pm 0.031$ a	0.704	$0.312\pm 0.016$ a	0.430	$0.312\pm 0.016$ b	0.303	$0.420\pm 0.021$ b	0.482
3	0.000	0.000	1.000	$41.00\pm 2.05$ a	41.580	$0.291\pm 0.015$ d	0.287	$0.625\pm 0.031$ a	0.648	$0.625\pm 0.031$ b	0.580	$0.312\pm 0.016$ b	0.317	$1.500\pm 0.075$ e	1.424
4	0.500	0.500	0.000	$44.12\pm 2.21$ b	43.460	$0.221\pm 0.011$ b	0.290	$0.625\pm 0.031$ a	0.556	$0.625\pm 0.031$ b	0.743	$0.312\pm 0.016$ b	0.266	$0.630\pm 0.032$ bc	0.601
5	0.500	0.000	0.500	$49.40\pm 2.47$ c	50.055	$0.221\pm 0.011$ b	0.240	$1.250\pm 0.063$ b	1.125	$1.250\pm 0.063$ c	1.108	$0.156\pm 0.008$ a	0.126	$0.510\pm 0.026$ bc	0.343
6	0.000	0.500	0.500	$40.00\pm 2.00$ a	39.500	$0.251\pm 0.013$ c	0.235	$0.625\pm 0.031$ a	0.727	$0.625\pm 0.031$ b	0.798	$0.156\pm 0.008$ a	0.156	$1.120\pm 0.056$ d	1.106
7	0.333	0.333	0.333	$49.28\pm 2.46$ c	48.012	$0.231\pm 0.012$ b	0.274	$0.625\pm 0.031$ a	0.830	$0.312\pm 0.016$ a	0.533	$0.312\pm 0.016$ b	0.200	$0.750\pm 0.038$ c	0.434
8	0.6667	0.1667	0.1667	$48.00\pm 2.40$ c	48.197	$0.310\pm 0.016$ e	0.265	$0.625\pm 0.031$ a	0.780	$0.625\pm 0.031$ b	0.747	$0.156\pm 0.008$ a	0.298	$0.135\pm 0.007$ a	0.513
9	0.1667	0.6667	0.1667	$39.80\pm 1.99$ a	44.265	$0.213\pm 0.011$ a	0.274	$0.625\pm 0.031$ a	0.604	$1.250\pm 0.063$ c	1.037	$0.156\pm 0.008$ a	0.214	$0.630\pm 0.032$ bc	0.550
10	0.1667	0.1667	0.6667	$47.20\pm 2.36$ c	45.880	$0.250\pm 0.012$ c	0.261	$0.625\pm 0.031$ a	0.775	$0.625\pm 0.031$ b	0.675	$0.156\pm 0.008$ a	0.172	$0.443\pm 0.022$ b	0.778

For each run, responses with different letters are significantly different (one-way analysis of variance, p < 0.05; Tukey’s test), ± standard deviation (SD) of three measurements

Exp experimental, Pred predicted