Figure 5.
miR-503 negatively regulates KIF5A to suppress T cell proliferation in contribution to immune escape of glioma through the IL-7 signaling pathway. (a) The expression of miR-503 and KIF5A in U-251 MG cells determined by RT-qPCR. (b, c) The protein expression of KIF5A and IL-7 in U-251 MG cells normalized to β-actin determined by Western blot analysis. (d) T cell proliferation detected by CFSE staining of flow cytometry. (e) T cell apoptosis detected by flow cytometry. (f) The expression of CD4 and FoxP3 determined by flow cytometry. *p < .05 vs. U-251 MG cells treated with mimic-NC + oe-NC. #p < .05 vs. U-251 MG cells treated with miR-503 mimic + oe-NC. The measurement data were expressed as mean ± standard deviation derived from at least 3 independent experiments. Data comparison was analyzed by one-way ANOVA among multiple groups, followed by Tukey’s post hoc test