FIGURE 1.

Primary amino acid sequences for rpM179 and the mechanistic probes SEKR, H2, H3, H8, and Delta. The amino acids that are removed from rpM179 to form the mechanistic probes are indicated by dashes. SEKR is formed by the removal of S16, E18, K24, and R31 in the N-terminus, H2 by the removal of H91 and H92, H3 by the removal of H67, H68, and H69, H8 by the removal of H58, H62, H67, H68, H69, H91, H92, and H99, and Delta by the removal of A167WPATDKTKREEVD180 in the C-terminus. Amino acids with side chains that are acidic, strongly basic, and weakly basic (histidines) are colored red, yellow, and purple, respectively. The serine at S16 that undergoes post translational phosphorylation is colored green. Note that the methionine at M1 is removed during the recombinant synthesis process.