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. 2022 Nov 22;7(22):e158060. doi: 10.1172/jci.insight.158060

Figure 4. PDOs grown in 2 different culture media show distinct transcriptomic signatures.

Figure 4

(A) Heatmaps showing clustering by differentially expressed genes for HO44 and HO163 PDOs cultured in WNT and PaTOM media with base mean ≥ 1, |fold change| > 2, and P value < 0.05; n = 2. (B) Gene sets enriched in PDAC PDO HO44 in WNT and PaTOM media using GSEA for Hallmark and KEGG gene sets. (C) Gene sets enriched in PDAC PDO HO163 in WNT and PaTOM media using GSEA for Hallmark and KEGG gene sets. NES, normalized enrichment score. (D) Summary of RNA-seq expression data for all 5 PDAC PDOs in WNT and PaTOM using GSEA Hallmark Gene Set categories; n = 5.