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. 2022 Nov 22;7(22):e152761. doi: 10.1172/jci.insight.152761

Figure 5. Neoadjuvant chemotherapy decreases the proportion of functionally exhausted CD4+ Tconv cells in PDAC.

Figure 5

t-SNE analysis based on intracellular cytokine expression of tumor-infiltrating CD4+ Tconv cells from patients who were primary resected (PR) (n = 8) and patients who received NEO (n = 8). (A) t-SNE analysis of CD4+ Tconv cells merged (left) and separated distribution from patients who were PR and patients who received NEO (right). (B) t-SNE expression of indicated cytokines. (C) FlowSOM clustering into 10 clusters (P1–P10). (D) Heatmap depicting mean fluorescence intensity for cytokine expression of each cluster (left), and bar graph showing distribution of each cluster within PR and NEO CD4+ Tconv cells (right). (E) Proportion of CD4+ Tconv cells within indicated clusters (PR vs. NEO). Each point represents data from 1 patient. Data are shown as the mean ± SEM. Unpaired 2-tailed t test. *P < 0.05; **P < 0.01.