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. 2022 Nov 22;7(22):e161870. doi: 10.1172/jci.insight.161870

Figure 5. Glutamic acid and aspartic acid induce vasodilation-related neuropeptides from peripheral neurons and keratinocytes.

Figure 5

(A) Immunoblot analysis of the VIP expression level in DRG neurons from glutamic acid gavage mice and control group. (B) Immunoblot analysis of the VIP expression level in DRG neurons from aspartic acid gavage mice and control group. See complete unedited blots in the supplemental material. (C and D) The mRNA expression levels of VIP, PACAP, CGRPα, and CGRPβ in HaCaT keratinocytes after glutamic acid or aspartic acid treatment (n = 3 for each group). (E and F) The mRNA (E) and protein (F) expression levels of VPAC2 in HDMECs after glutamic acid or aspartic acid treatment (n = 3 for each group). All results are representative of at least 3 independent experiments. Data represent the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. One-way ANOVA with Bonferroni’s post hoc test was used.