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. 2022 Nov 22;7(22):e138539. doi: 10.1172/jci.insight.138539

Figure 10. Human monocyte cholesterol and inflammation.

Figure 10

(A) Monocyte cholesterol levels correlated positively with carotid IMT in humans. (B) Human CD14+ monocytes were treated for 20 hours with PRX (2 mM βCD) or vehicle prior to the 4-hour LPS stimulation. Localization of free cholesterol (visualized with filipin) and LAMP1 was assessed using confocal microscopy. Scale bar, 3 μm. (C) Human CD14+ monocytes were treated for 20 hours with PRX prior to 4 hours with LPS. IL6 and IL1B mRNAs were quantified using qPCR. n = 3. *P < 0.05 vs. LPS-untreated cells, #P < 0.05 vs. LPS only–treated cells, Tukey-Kramer post hoc test. Data shown as mean ± SD in all panels where P values are shown.