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. 2022 Nov 22;7(22):e138539. doi: 10.1172/jci.insight.138539

Figure 8. PRX suppresses inflammatory responses and modulates cholesterol metabolism in BMDMs.

Figure 8

(A) BMDMs were treated with or without PRX (2 mM βCD) for 20 hours followed by LPS or vehicle for 4 hours. Filipin and LAMP1 staining are shown. Scale bar, 5 μm. (B) GSEA enrichment score for LXR target genes in PRX-treated BMDMs as compared with untreated BMDMs. (C and D) RAW cells were treated for 20 hours with or without PRX (2 mM βCD) prior to 4 hours with LPS. Cellular levels of desmosterol (C) and 25-hydroxycholesterol (D) were assessed by GC/MS. *P < 0.05 vs. untreated cells, #P < 0.05, Tukey-Kramer post hoc test. (E) Expression of Hmgcr and Hmgcs1 mRNA in RAW cells. *P < 0.05, Student’s 2-tailed t test. Data shown as mean ± SD in all panels where P values are shown. (F and G) GSEA enrichment scores in PRX-treated BMDMs as compared with untreated BMDMs. The SREBP1 target gene set consisting of the genes with SREBP1 peaks in their regulatory regions (F) and that for hallmark cholesterol homeostasis (G) were analyzed.