Figure 8. Effect of HCO₃^– transport and CFTR rescue on airway surface properties.

The graphs show the results of FRAP experiments on bronchial epithelia that were pretreated for 72 hours with/without IL-17/TNF-α. (A) Data obtained in non-CF and CF bronchial epithelia with HCO₃^– or Hepes buffered media on the basolateral side (for 3 hours before measurements). (B) Data from experiments in non-CF and CF bronchial epithelia with YS-01 (5 μM) added to the basolateral side for 3 hours before measurements. (C) Results from F508del/F508del CF bronchial epithelia. Where indicated, epithelia were treated with the VX-809 (1 μM) plus VX-445 (5 μM) or vehicle in the last 24 hours before measurements. All epithelia were stimulated with isoproterenol (100 nM, basolateral). *P < 0.05; **P < 0.01; and ***P < 0.001 (Kruskal-Wallis and Dunn’s test). (D) The illustration shows the proposed changes in ion transport that occur when epithelia treated with IL-17/TNF-α are stimulated with the β-adrenergic agonist.