Figure 3.

Reporter assays ascribe selective regulatory function to cg17589883 region in MUC5B. (A) UCSC Genome Browser screenshot of the MUC5B locus with H3K27Ac Mark, DNase I Hypersensitivity Peak, and Transcription Factor (TF) ChIP-seq Cluster tracks from The Encyclopedia of DNA Elements Consortium enabled and used to identify regions with putative regulatory function for cloning into luciferase reporters, as indicated. (B) Mean ± SD normalized luciferase activity of indicated reporter constructs and empty vector (EV) control in A549 cells under basal culture conditions (n = 4 per group and *P < 0.0001 versus EV via one-way ANOVA with Bonferroni correction). ANOVA = Analysis of Variance; Reg = region.