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. 2022 Dec 8;7(23):e162392. doi: 10.1172/jci.insight.162392

Figure 1. BLT1 functions as a major epithelial receptor for RvE1.

Figure 1

(A) RNAscope staining for LTB4R and CMKLR1 mRNA expression in frozen sections from colonic tissue of humans. (B) RNAscope staining for Ltb4r1 and Cmklr1 mRNA expression in frozen sections from colonic tissue of mice. Scale bars: 50 μm. (C) qPCR analysis of the expression of CMKLR1 and LTB4R mRNA in the SKCO-15, T84, and human 2D colonoids. The data are presented as the mean ± SEM. Cq, quantification cycle (measured as cycles). (D) Effect of BLT1 antagonist on the prorepair activity of RvE1 in the scratch wound assay using human primary IECs. After scratch wound was produced, IECs were incubated with RvE1 (100 nM) for 24 hours. BLT1 (CP105,696; 1 μM) or CMKLR1 (α-NETA; 10 μM) antagonist was applied 30 minutes before RvE1 treatment. Quantification of wound repair at 24 hours after wounding is shown. The data are presented as the mean ± SEM. Statistical analysis was performed using 1-way ANOVA followed by post hoc Welch’s t test with Bonferroni’s correction. *P < 0.05, **P < 0.01, compared with RvE1.