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. 2022 Dec 8;7(23):e162392. doi: 10.1172/jci.insight.162392

Figure 2. Epithelial BLT1 is upregulated in response to colonic mucosal injury.

Figure 2

(A) The changes in the expression of Ltb4r1 mRNA in 3 mm punch biopsies of intact colonic tissues and colonic mucosal wounds on different days after injury. The data are presented as the mean ± SEM of 4–5 mice. Statistical analysis was performed using 1-way ANOVA followed by post hoc Welch’s t test with Bonferroni’s correction. *P < 0.05, **P < 0.01, compared with intact tissue (IT). (B–D) RNAscope staining for Blt1 mRNA in frozen sections from intact tissues and wounded colonic tissues 2 days after injury. Arrows indicate upregulation of Ltb4r1 expression in the crypts next to the wound. W, wound. Scale bar is 50 μm. (E) The number of Ltb4r1 mRNA–positive dots in the crypt of intact colonic tissues and colonic mucosal wounds (adjacent to wound) 2 days after injury is shown. The data are presented as the mean ± SEM of 6 mice. Statistical analysis was performed using an unpaired (2-tailed) t test with Welch’s correction. *P < 0.05, compared with IT. AW, adjacent to wound. (F and G) RNAscope staining for LTB4R mRNA expression in frozen sections from healthy controls and patients with ulcerative colitis.