Figure 5. ASO-mediated knockdown of Kcnt1 at P40 improves the behavioral phenotype of adult L/L mice.

(A) Total ambulatory distance was reduced in mice treated with Kcnt1 ASO 500 μg compared with control-treated mice (P = 0.0002) but not for mice treated with ED₅₀ or ED₈₀ (P = 0.775 and 0.839, respectively) (Kruskal-Wallis test, followed by Dunn’s multiple comparisons, +/+ n = 12, untreated n = 25, control ASO n = 13; ED₅₀ n = 13; ED₈₀ n = 10, 500 μg n = 11). (B) Time spent in the light compartment during the light/dark box test (Kruskal-Wallis test, followed by Dunn’s multiple comparisons P = 0.588; +/+ n = 12, L/L n = 17, control ASO n = 11, ED₅₀ n = 13, ED₈₀ n = 10, 500 μg n = 12). (C) Time spent in the open arms of the EPM (Kruskal-Wallis test, followed by Dunn’s post hoc analysis; +/+ n = 10; untreated n = 15; control ASO n = 9; ED₅₀ n = 13; ED₈₀ n = 10, 500 μg n = 12). (D) Time spent in the novel arm of the Y maze (Kruskal-Wallis test, followed by Dunn’s post hoc analysis P = 0.551; +/+ n = 8; untreated n = 10; ED₅₀ n = 12; ED₈₀ n = 10, 500 μg n = 10).