TABLE 1.
Phagocytic particle | Time after uptakeb | % Cells of categoryc:
|
|||
---|---|---|---|---|---|
1 | 2 | 3 | 4 | ||
None | NAd | 18 | 82 | 0 | 0 |
B. subtilis | 45 min | 20 | 80 | 0 | 0 |
Latex beads | 2 h | 18 | 82 | 0 | 0 |
M. smegmatis | 1 day | 23 | 77 | 0 | 0 |
M. avium | 2 h | 24 | 76 | 0 | 0 |
1 day | 0 | 21 | 79 | 0 | |
2 days | 0 | 2 | 38 | 60 | |
6 days | 0 | 0 | 0 | 100 |
For each particle and time point, counts were made on Mφs in triplicate monolayers. Each value represents the mean of three determinations on 200 cells each. The standard error of the mean was between 5 and 10%. Data are from a typical experiment.
Uninfected cells were cultured in parallel to M. avium-infected ones, and counts were done at the same time points for both cases. The data were taken on day 6. Identical results were obtained at all other time points.
Categories were defined according to the morphological state and distribution of actin filaments (Fig. 1).
NA, not applicable.