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. Author manuscript; available in PMC: 2022 Dec 13.
Published in final edited form as: Cell. 2022 Sep 27;185(21):3950–3965.e25. doi: 10.1016/j.cell.2022.09.019

Figure 4. Inactive-state binding cyclic peptide GD20 is a Gαs specific guanine nucleotide dissociation inhibitor.

Figure 4.

(A) Illustration of inactive-state binders inhibiting Gαs steady-state GTPase activity.

(B) Gαs steady-state GTPase activity was inhibited by GD peptides. The data represent one measurement. Gαs steady-state GTPase activity in the presence of GD20 was repeated twice in Figure S4A.

(C) Structure of the resynthesized cyclic peptide GD20.

(D) GD20 inhibited Gαs GDP dissociation. Mean ± SD, n = 3.

(E) GD20 inhibited GTPγS binding to Gαs. Mean ± SD, n = 3.

See also Figure S4.