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. 2022 Dec 6;2022:5184721. doi: 10.1155/2022/5184721

Figure 4.

Figure 4

Effects of amentoflavone on regulating the TLR4/MyD88/NF-κB axis to alter LPS-induced proinflammatory cytokine levels. (a) Representative immunoblots of TLR4, MyD88, p-IκB, IκB, and NF-κB p65 proteins in the cytoplasm and cytomembrane in BV2 microglial cells. (b) Representative immunoblots of NF-κB p65 proteins in the nucleus in BV2 microglial cells. (c, d) Semiquantitative analysis of the relative levels of TLR4, MyD88, p-IκB, IκB, and NF-κB p65 in (a) by densitometric analysis. (e) Semiquantitative analysis of the relative levels of NF-κB p65 in (b) by densitometric analysis. Error bars represent mean ± s.d. ∗∗ and ∗∗∗ represent P < 0.01 and P < 0.001, respectively. All experiments were performed in triplicate. LPS: lipopolysaccharide; AF: amentoflavone.