TABLE 1.
SI of lymph node cells from mice immunized with an extract of P. falciparum and then incubated in vitro with peptides and controls
Peptide | SI for mouse straina:
|
|||||
---|---|---|---|---|---|---|
C57BL/10
|
B10.BR
|
BALB/c
|
||||
1 μMb | 5 μMb | 1 μM | 5 μM | 1 μM | 5 μM | |
MSP-1#1 | 1.8 ± 0.4 | 1.5 ± 0.2 | 1.0 ± 0.2 | 0.6 ± 0.3 | 1.0 ± 0.1 | 1.6 ± 0.4 |
MSP-1#2 | 1.9 ± 0.2 | 1.6 ± 0.2 | 1.2 ± 0.3 | 1.1 ± 0.3 | 0.9 ± 0.1 | 1.8 ± 0.3 |
MSP-1#3 | 1.4 ± 0.4 | 1.4 ± 0.1 | 1.1 ± 0.4 | 0.8 ± 0.4 | 1.1 ± 0.2 | 1.1 ± 0.2 |
MSP-1#4 | 2.1 ± 0.4 | 1.4 ± 0.1 | 1.8 ± 0.2 | 1.2 ± 0.2 | 1.2 ± 0.1 | 2.1 ± 0.3 |
MSP-1#5 | 1.5 ± 0.4 | 1.2 ± 0.4 | 0.9 ± 0.2 | 0.7 ± 0.4 | 0.6 ± 0.1 | 1.3 ± 0.1 |
MSP-1#23 | 1.6 ± 0.3 | 1.6 ± 0.1 | 1.4 ± 0.1 | 0.9 ± 0.1 | 0.9 ± 0.2 | 1.5 ± 0.2 |
PPD | 4.4 ± 1.0 | 4.2 ± 1.2 | 9.4 ± 3.2 | |||
RBC | 2.7 ± 0.6 | 2.2 ± 0.3 | 2.4 ± 0.8 | |||
Extractc | 43 ± 1.9 | 42.8 ± 4.1 | 37.9 ± 7.4 |
Results are expressed as mean SI ± standard error of the mean from two to four experiments. Positive responses are shown in bold type.
Concentration of peptide used in vitro.
P. falciparum merozoite extract.