Table 2. Fatty acid composition strongly impacted by CF.
| Fatty acid | Tissue | Class | Mole% | Age | Species | N | P-value | |
|---|---|---|---|---|---|---|---|---|
| Non-CF | CF | |||||||
| 16:0 | Liver | L | 20.7 ± 0.4 | 18.5 ± 0.3 | Newborn | Pig | 7.13 | 9E−05 |
| 16:1n7 | Liver | PC | 4.0 ± 0.3 | 5.7 ± 0.2 | Newborn | Pig | 7.13 | 9E−05 |
| 18:1* | Plasma | LPE | 20.4 ± 3.5 | 49.5 ± 1.8 | Newborn | Ferret | 5.5 | 8E−05 |
| 18:2n6 | Liver | PC | 9.4 ± 1.2 | 4.6 ± 0.2 | Newborn | Pig | 7.13 | 3E−05 |
| Liver | PE | 4.7 ± 0.6 | 1.9 ± 0.1 | Newborn | Pig | 7.13 | 8E−06 | |
| Serum | PL | 15.9 ± 1.6 | 4.0 ± 0.4 | Newborn | Pig | 7.13 | 2E−08 | |
| 20:1* | Plasma | NEFA | 0.6 ± 0.1 | 1.4 ± 0.1 | Newborn | Ferret | 5.5 | 7E−05 |
| 20:2n6 | Liver | PC | 0.2 ± 0.0 | 0.1 ± 0.0 | Newborn | Pig | 7.13 | 5E−05 |
| Liver | PE | 0.2 ± 0.0 | 0.1 ± 0.0 | Newborn | Pig | 7.13 | 1E−05 | |
| Serum | PL | 0.2 ± 0.0 | 0.1 ± 0.0 | Newborn | Pig | 7.13 | 6E−06 | |
| 20:3n6 | RBC | L | 0.4 ± 0.0 | 0.9 ± 0.0 | Juvenile | Ferret | 6.5 | 1E−06 |
| 20:4n6 | Liver | PE | 24.4 ± 0.7 | 29.3 ± 0.4 | Newborn | Pig | 7.13 | 2E−06 |
| 24:1n9 | RBC | L | 1.2 ± 0.0 | 2.1 ± 0.1 | Juvenile | Ferret | 6.5 | 2E−06 |
*Unsaturated fatty acids without an omega indicator were measured by mass spectrometry and thus positional information was not determined. Species 18:1 is a mixture of different omega isomers, typically mostly 18:1n9 but containing varying relative amounts of 18:1n7. By contrast, species 20:1 is nearly exclusively isomer 20:1n9.
Of 559 pairwise CF versus non-CF comparisons, 13 met statistical significance accounting for multiple hypothesis testing, with P<0.00009. Lipid classes: L, total lipid; LPE, lysophosphatidylethanolamine; NEFA, non-esterified fatty acids; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PL, all phospholipids. N is the number of non-CF and CF animals examined.