Table 1.
Bioanalytical method summary for the analyte DRV and the internal standard RTV.
| Optimized mass parameters | |||
|---|---|---|---|
| Analyte | Transitions (m/z) | CE (eV) | CXP (eV) |
| Darunavir (DRV) | 547.7/392 | 20 | 30 |
| Ritonavir |
721.3/296.1 |
27 |
29 |
| Other parameters: DP: 100, EP: 10, CUR: 20, CAD: 8, IS: 5500, GS1: 50, GS2: 50, Temp: 500 | |||
| Liquid chromatography conditions | |||
| HPLC Column: Waters Xterra® MS C18 Column, 125 Å, 3.5 μm,4.6 mm × 50 mm | |||
| Mobile Phase: acetonitrile and water with 0.1% formic acid Injection volume: 1 μl | |||
| Total flow: 1 ml/min | |
|---|---|
| Gradient Time | Organic solvent (%) |
| 0.10 | 30 |
| 1.00 | 50 |
| 1.50 | 60 |
| 5.00 | 60 |
| 5.10 | 30 |
MS parameters are abbreviated as follows: Capillary electrophoresis (CE), Cell exit potential (CXP), Declustering potential (DP), Entrance potential (EP), Curtain gas (CUR), Collision gas (CAD), Ion spray velocity (IS), Ion source gas 1 (GS1), Ion source gas 2 (GS2), Temperature (Temp).