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. 2022 Dec 13;12:21496. doi: 10.1038/s41598-022-25405-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PARP1^+/+ and PARP1^−/− mice pancreas comparative histology, islet quantification, and immune characterization. (a) Represents H&E histology of mice pancreas from 129SV-B6 stain PARP1 wild type and PARP1 KO mice. Arrow represents islets, and outline shows a region of ductal fibrosis. (b) Depicts pancreatic islet histology between PARP1 wildtype and KO mice and quantification of mean islet area represented as % of the total pancreas (n = 4). (c, d) Show quantification for mean islet size and number distribution in the pancreas of both genotypes. To assess this, harvested pancreas from 4 animals in each genotype were assessed. Tissue slides from each animal were stained; 3 slides per section from each of the 4 animals were imaged and quantified. Data represented as mean ± sem, p-value show significance at 95% confidence interval using ttest analysis compared to wildtype animals (n = 4). (e) Shows the distribution of endocrine fraction within the pancreas between wildtype and PARP-KO mice representing % islet fraction (distribution per unit area) relative to total pancreas area (n = 4). (f) Represents immunohistochemistry images for pancreatic progenitor markers- pdx1 (green), nestin (green), ngn-3 (red) and insulin (red). (g) Shows brightfield phase contrast microscopic images of PREP cells isolated from wildtype and KO mice pancreas with immunocytochemistry for progenitor markers- pdx1 (green), nestin (green), ngn-3 (red) and insulin (red).