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. 2022 Dec 13;12:21496. doi: 10.1038/s41598-022-25405-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Creation of PARP1^+/+ (U6) and PARP1^−/− (SiP) cells for functional characterization. (a) Schematic representation for clone generation, selection, and isolation after PARP1 silencing using SiP and mock U6 shRNA control. Cells for experimentation were confirmed for absolute PARP1 depletion at passage 5. (b) Immunoblot confirmation of PARP-1 depletion in SiP and U6 cells. The graph represents quantification for PARP1 depletion in most established clones after 3 independent passages. Data represented as mean ± sem, p-value show significance at 95% confidence interval using ttest analysis compared to U6 cells (n = 3). (c) Phase contrast images for PARP1 control and deplete SiP-PANC-1 cells showing morphological changes after KD. Fluorescent staining show images for PARP-1 (green). (d) Immunocytochemistry images for pancreatic progenitor- nestin (red), pdx1 (green), and ngn-3 (red) in PANC-1 U6 and SiP cells.