Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 13;12:21496. doi: 10.1038/s41598-022-25405-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Islet cell differentiation and characterization for insulin production in PARP1 control and deplete PANC-1 cells. (a) Phase contrast microscopic images of PANC-1 U6/SiP cells differentiated with control (SFM) and activin-A on day 10th. Color brightfield images show the presence of brick-red insulin staining with dithizone (DTZ) after differentiation. (b) Graph show quantification for islet-like clusters generated per field of an image from PANC-1 U6/SiP cells at day 10th differentiation. (c) Immunocytochemical characterization of generated clustered from PANC-1 U6/SiP cells for islet cell markers- insulin and glucagon (green). (d) Quantification for mean fluorescence intensity for human insulin production in differentiated U6 and SiP cells. Data represented as mean ± sem, p-value show significance at 95% confidence interval using two-way anova analysis compared to U6 cells (n = 3). (e) Quantification and modelling for presence of insulin in U6 vs SiP cells using 2-dimensional mean fluorescent intensity dot plot representation extracted from immunostained images with Axio-vision Zen 10 software, Zeiss, Canada, where insulin stays on Y-axis and dapi is marked on x-axis.