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Summarizes details of RT-PCR primer sequences and PCR conditions for islet cell differentiation markers.
| Gene ID | PRIMER SEQ-Forward | PRIMER SEQ-Reverse | Tm | Size (bp) |
|---|---|---|---|---|
| Insulin | GCCCAGGCTTTTGTCAAACA | CTCCCCACACACCAGGTAGAG | 55 | 90 |
| Glucagon | ATGAAGACCATTTACTTTGTGGCT | GGTGTTCATCAACCACTGCAC | 58 | 243 |
| β-Actin | GGACTGTTACTGAGCTGCGTT | CGCCTTCACCGTTCCAGTT | 57 | 209 |
