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. 2022 Dec 13;12:21570. doi: 10.1038/s41598-022-25065-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Combination therapy efficiently enhances dendritic cell activity. Mice were inoculated with Pan02 tumors as indicated in Fig. 1a, and tumors and TDLNs were collected as previously shown. (a) Bar graphs show the percentage of DCs (MHC-II⁺ CD11c⁺) on the injected and contralateral sides. (b) Bar graphs show the percentage of MHC-I expression on DCs on the injected and contralateral sides. (c) A representative dot plot shows cDC1 (CD103⁺ CD11b^low) from cDCs (MHC-II⁺ CD11c⁺ CD8α⁺) on the injected and contralateral sides. (d) Bar graphs show the percentage of cDC1 from cDCs on the injected and contralateral sides. (e) Representative histograms show XCR-1 expression on cDC1 on the injected and contralateral sides. (f) Bar graphs show the percentage of XCR-1 expression on cDC1 on the injected and contralateral sides. (g) A bar graph shows the percentage of cDC1 from DCs in TDLNs. (h) A bar graph shows the percentage of XCR-1 expression on cDC1 in TDLNs. Data are presented as mean ± SD (n = 4 mice). This experiment was conducted at least two times. One-way ANOVA with a post-hoc Tukey’s tests was performed. * p < 0.05, ** p < 0.01, *** p < 0.001.