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. 2022 Sep 23;45(6):1237–1251. doi: 10.1007/s13402-022-00713-5

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© The Author(s) 2022, corrected publication 2022

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Fig. 2 — Expression of ABCA6 influences malignancy and chemosensitivity of EWS cells. a, ABCA6 mRNA relative expression by RT-qPCR. Data are the mean ± SEM (n = 3) (left). Protein expression of ABCA6 by western blotting. GAPDH was used as a loading control (right). b, Colonies formation in anchorage-independent conditions of PDX-EW#5-C and PDX-EW#3-C (ABCA6^high) and PDX-EW#2-C and PDX-EW#4-C (ABCA6^low). Pictures are from a representative experiment (40X magnification). Data are the mean ± SEM (n = 3). c, Migratory abilities of PDX-EW#5-C and PDX-EW#3-C (ABCA6^high) and PDX-EW#2-C and PDX-EW#4-C (ABCA6^low). Data are the mean ± SEM (n = 6). d, Mitochondrial depolarization after cell exposure to doxorubicin (DXR, 24 h) detected by flow cytometry. A dose-dependent increase in mitochondrial depolarization was observed. Data are the mean ± SEM (n = 2). Drug effects were significantly higher in PDX-EW#5-C cells (ABCA6^high) than in the PDX-EW#2-C cells (ABCA6^low) (****p < 0.0001, two-way ANOVA). Efficacy of doxorubicin in each cell line compared to respective control was tested by one-way ANOVA, reporting significant p values (range from p < 0.05 to p > 0.0001). e, Protein expression of cleaved caspase 3 and PARP after cell exposure to DXR (24 h) by western blotting. GAPDH was used as a loading control