Fig. 3.

Forced expression or silencing of ABCA6 affects cell migration and chemosensitivity to doxorubicin. a, Expression of ABCA6 in PDX-EW#2-C cells after forced expression (left) or in PDX-EW#5-C cells after silencing (right) determined by western blotting. A representative experiment of three is shown. GAPDH was used as a loading control. b, Migratory ability of PDX-EW#2-C and PDX-EW#5-C transfected cells. Data are the mean ± SEM (n = 3); *p < 0.05, **p < 0.01, one–way ANOVA vs control (non-transfected cells; NT). c, Sensitivity to doxorubicin (DXR) of transfected cells, expressed as IC50 values, after 24 h of treatment. Data are the mean ± SEM (n = 3); **p < 0.01 one–way ANOVA vs control (non-transfected cells; NT). d, Mitochondrial depolarization after cells exposure to DXR (24 h) detected by flow cytometry. Sensitivity to DXR of control cells was compared to that of ABCA6 overexpressing (hABCA6) or ABCA6 silenced cells (shABCA6). Data are the mean ± SEM (n = 3). ***p < 0.001, two-way ANOVA