Figure 4.

Result of the Western blot analysis using the recombinant R2 fragment of the glycoprotein B of PCMV/PRV. (A) nine weeks old animals (number 1 to 10) with positive PCR results in at least one tissue (Fig. 1, Supplementary Table 1) and (B) the pigs U, V, and W, 43 or 41 weeks old and which were PCR-negative (Table 2). P, positive control, N, negative control. Serum dilution 1:300, goat anti-pig horse reddish peroxidase was used for detection. Blots were cut prior to hybridization with antibodies during blotting, each stripe was incubated with an individual serum.