Table 1.
Primers and probes used in this study.
| PCR assay | Primer/probe | Sequence 5ʹ-3ʹ | Amplicon (base pair) | References |
|---|---|---|---|---|
| PCMV 1 | PCMV-Fwd | ACT TCG TCG CAG CTC ATC TGA | 63 | 2,17,30* |
| PCMV -Rev | GTT CTG GGA TTC CGA GGT TG | |||
| PCMV -Probe | 6FAM-CAG GGC GGC GGT CGA GCT C-BHQ | |||
| PCMV 2 |
Fr-t (nt279-299)** Rr-t (nt373-354)** Probe (nt331-350)** |
AAT GCG TTT TAC AAC TTC ACG CTG AGC ATG TCC CGC CCT AT 6FAM-CTCTAGCGGCGTCCATCACC-BHQ |
31 | |
| pGAPDH | pGAPDH-Fwd | ACA TGG CCT CCA AGG AGT AAG A | 106 | 32 |
| pGAPDH-Rev | GAT CGA GTT GGG GCT GTG ACT | |||
| pGAPDH-Probe | HEX-CCA CCA ACC CCA GCA AGA GCA CGC-BHQ1 |
*The PCMV real-time PCR was modified and performed as duplex PCR.
**nt, nucleotide, GenBank AJ222640.
PCR polymerase chain reaction, PCMV porcine cytomegalovirus, pGAPDH porcine glyceraldehyde-3-phosphate dehydrogenase, Fwd forward primer, Rev reverse primer, nt nucleotide, 6FAM 6-Carboxyfluorescein, BHQ black hole quencher, HEX Hexachloro-fluorescein.