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. 2022 Dec 13;13:7728. doi: 10.1038/s41467-022-35509-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The induction of HTR5 by estrogen during imbibition. Seeds were imbibed with or without estrogen for 48 h. Values are means ± SD of three biological replicates. PP2A was used as an endogenous control for normalization. b The germination phenotype of h3.3ko/+;pER8::HTR5 progenies treated with or without estrogen during imbibition. Arrows indicate h3.3ko;pER8::HTR5 seeds that are strongly delayed in germination. c Percentages of h3.3ko;pER8::HTR5 seeds that germinated within 2 month of imbibition with or without estrogen. Values are means ± SD of three biological replicates. 50 seeds were assessed for each replicate. d Heatmap showing the transcript levels of H3.1 and H3.3-coding genes in dissected embryos at different (early/middle/late) developmental stages. DAP: day after pollination. The expression data were extracted from the published RNA-seq datasets⁴⁵. e The germination phenotypes of Col, h3.3ko, and h3.3ko;pH2B.S::HTR5 after subjected to germination for 84 h. f Percentages of h3.3ko;pH2B.S::HTR5 seeds that germinated within 10 days of imbibition. Values are means ± SD of three biological replicates. At least 38 seeds were assessed for each replicate. g Metaplot of HTR5-GFP ChIP-seq signals over all genes in mature seeds, during imbibition, and in the 10-day-old seedling. TSS: transcription start sites, TES: transcription end sites. h Heatmap of HTR5-GFP ChIP-seq signals over all genes in mature seeds, during imbibition, and in the 10-day-old seedling.